Search results for " tangential flow"
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Exosome isolation by tangential flow filtration and size exclusion chromatography
The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for the concentration of exosomes followed by gentle size exclusion chromatography (SEC) with our next-…
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Achieving Process Balance with Perfusion Bioreactors
Alternating tangential flow (ATF) filtration technology (developed by Refine Technology, now Repligen) is commonly used.
For perfusion processes used to manufacture chimeric antigen receptor (CAR)…
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Tools for Continuous Bioprocessing Development
For example, perfusion process development experiments (21) using a 4-L working-volume Wave bag and alternating tangential flow (ATF) or tangential flow filtration (TFF) cell-retention filter, consume…
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Digitalization: The Route to Biopharma 4.0
For formulation, a model for predicting volumetric concentration factor across single pass tangential flow ultrafiltration (SPTFF) can be used to develop real-time automated monitoring and a control s…
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Continuous Processing for the Production of Biopharmaceuticals
The authors review the technologies that may help bioprocessing become a truly continuous operation and present case studies that could contribute to the integration of upstream and downstream platf…
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Updating Viral Clearance for New Biologic Modalities
In terms of traditional viral clearance products, Verlenden continues, studies have shown that concentrating protein solutions using single pass tangential flow filtration (SPTFF) before loading them …
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Other processes that are being utilized include tangential flow filtration that takes advantage of the large particle size for separation from smaller cellular contaminants.
VACCINES
Vaccines…
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Design Considerations for a Commercial Cell and Gene Therapy Facility
For mAb bioprocessing and viral-vector manufacturing, the equipment needed is similar, such as single-use bioreactors and standard harvest and downstream systems for clarification, tangential flow fil…
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Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…
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Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the added benefits of the single‑use technology and consistency through automation.
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